Hi just wondering if you have any experience with this problem.
I am following GATK best practices for a targeted sequencing experiment.
After the GenotypeGVCFs phase the majority of variants are marked "MQ=NaN"
Inspection of g.vcf reveals that the only sites which have a numerical MQ are those which are homozygous for the alternative allele
I've included below the three commands used to get to that point
I have tried this with and without -A AS_RMSMappingQuality
Thanks so much for your help!
for filename in *.bam; do $gatk -T HaplotypeCaller -nct 15 -R ucsc.hg19.fasta -I ${filename} -L bedfile.bed -ERC GVCF -o ${filename}.g.vcf;done
ls *.g.vcf > gee_vee_cee_eff.list
$gatk -T GenotypeGVCFs -R ucsc.hg19.fasta -nt 15 -V gee_vee_cee_eff.list -o GVCFs_jointcalls.vcf