Quantcast
Channel: haplotypecaller — GATK-Forum
Viewing all articles
Browse latest Browse all 1335

calling variants on haploid organism

$
0
0

Hi,

I'm curious about something I find using HaplotypeCaller on my haploid fungus. (using it with -ploidy 1)
In my vcf there are some variants with low variant allele frequency (around 50%).

for example : chr_5 522158 . C T, 34.01 . BaseQRankSum=0.529;ClippingRankSum=0.689;DP=15;MLEAC=1,0;MLEAF=1.00,0.00;MQRankSum=-0.380;RAW_MQ=18495.00;ReadPosRankSum=0.831 GT:AD:DP:GQ:PL:SB 1:5,7,0:12:64:64,0,228:3,2,3,4

Are there no filters for those kind of variants when using -ploidy 1 option? (for an haploid species I'm not supposed to find both ref and alt on the same position)

Thank you for your help,

Guillaume


Viewing all articles
Browse latest Browse all 1335

Trending Articles