Hi everyone,
I got the following warning when running the HaplotypeCaller.
BAM index file M11.bai is older than BAM M11.bam
Although the warning presents in the log, it also gives me a gvcf. Does this warning effect the accuracy of the result? I hope this warning will be fine for me.
I declare those bam files were produced on another server.
The command
java -Xmx50g -Djava.io.tmpdir=/data/tmp -jar /bin/GATK3.5/GenomeAnalysisTK.jar \
-R ref.fa \
-T HaplotypeCaller -nct 8 \
-I ./$d'.bam' \
-o $d'.g.vcf' \
--genotyping_mode DISCOVERY \
-stand_emit_conf 30 \
-stand_call_conf 30 \
-ERC GVCF
done
What is more, I have another unsure on the new version highlights.
When I run above command with parameters -variant_index_type LINEAR -variant_index_parameter 128000
, Do they will come to the same results?
Many thanks!