I have processed samples using GATK 3.1 Haplotypecaller according to the RNA Seq best practice. Haplotypecaller is set with --stand_emit_conf = 20 and --stand_call_conf = 20 options, but I could find variants which QUAL less than 20 in the output gVcf file and variants which its QUAL < 20 is not marked as LowQual. I wonder what those parameters do for Haplotypecaller.
I have run with --stand_emit_conf = 20 and --stand_call_conf = 30 for testing. Output gvcf file appears to be identical.
Thanks!